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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in advertising and marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been explained. Two added splice variants had been claimed but in-depth nucleotide sequence data is not really nevertheless accessible.
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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide normally contains the epitope acknowledged by the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the best possible benefits are...
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This information outlines even further doable optimization of mobile and immunoassay parameters to guarantee the very best benefits are...
Antibodies certain to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining here with the blocked antibody as opposed to the antibody on your own, one can see which staining is particular.
Target Information and facts Cyclin E belongs into the really conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance from the mobile cycle. Cyclins functionality as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead for the temporal coordination of each mitotic party. Cyclin E forms a complex with and capabilities for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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